| 3种海藻凝集素组分及糖抑制作用的初步研究 |
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| 引用本文： | 郑怡,余萍,刘燕如. 3种海藻凝集素组分及糖抑制作用的初步研究[J]. 植物资源与环境学报, 2002, 11(4): 19-21 |
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| 作者姓名： | 郑怡 余萍 刘燕如 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福建,福州,350007
2. 福建师范大学高分子研究所,福建,福州,350007 |
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| 基金项目： | 福建省自然科学基金资助项目 (B0 110 0 0 9) |
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| 摘 要： | 从 3种海藻 (铁钉菜IshigeokamuraeYenda、坛紫菜PorphyrahaitanensisChangetZheng和脆江蓠Gracilariabursa pastorisSilva)中分离纯化得到铁钉菜凝集素 (IOL)、坛紫菜凝集素 (PHL)及脆江蓠凝集素 (GBL)。经测定 ,它们分别含有 7%、14 %和 11%的中性糖。氨基酸组成中 ,苯丙氨酸含量最高 ,蛋氨酸和精氨酸次之。 3种海藻凝集素的凝血活性均可被单糖或双糖所抑制 ,在所测试的 13种糖类中 ,能够抑制GBL活性的糖类最多 ,达 5种 ,抑制IOL的糖类最少 ,仅 2种。 3种糖蛋白 (胃粘蛋白、卵粘蛋白和甲状腺球蛋白 )对IOL没有抑制作用 ,而卵粘蛋白能够抑制PHL和GBL的凝血活性
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| 关 键 词： | 海藻 凝集素 组分 糖 |
| 文章编号： | 1004-0978(2002)04-0019-03 |
| 修稿时间： | 2002-05-31 |
Preliminary studies on component and sugar inhibition of three marine algal lectins |
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| ZHENG Yi {},YU Ping {},LIU Yan|ru {}. Preliminary studies on component and sugar inhibition of three marine algal lectins[J]. Journal of Plant Resources and Environment, 2002, 11(4): 19-21 |
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| Authors: | ZHENG Yi {} YU Ping {} LIU Yan|ru {} |
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| Affiliation: | ZHENG Yi {1},YU Ping {2},LIU Yan|ru {1} |
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| Abstract: | Three kinds of algal lectin (IOL, PHL and GBL) were extracted and purified from three marine algae (%Ishige okamurae %Yenda, %Porphyra haitanensis %Chang et Zheng and %Gracilaria bursa|pastoris %Silva). They contain 7%, 14% and 11% neutral saccharide, respectively. In their amino acid composition, Phe has the highest content, followed by Arg or Met. Agglutinic activities of these lectins were inhibited with the presence of some mono| or bi|saccharides. In sugar inhibition, there were the most numbers of sugars which inhibited the agglutinic activity of GBL and the fewest numbers of sugars which inhibited the agglutinic activity of IOL. Three kinds of glycoproteins (Mucin, Ovalbumin and Thyroglobuin) did not inhibit the activity of IOL, but Ovalbumin showed inhibition for the activities of PHL and GBL. |
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| Keywords: | marine algae lectin component sugar |
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