Short Nissl Staining for Incubated Cryostat Sections of the Brain |
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Authors: | O F C Lindroos |
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Institution: |
a Department of Physiology, University of Helsinki, Helsinki, Finland |
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Abstract: | Nissl stain often binds poorly to cryostat sections which have been incubated in solutions of radiolabeled ligands. Such incubation is used in receptor autoradiography of the brain when using the in vitro method. We have developed a rapid (16 min) modification of Nissl staining for sections that bind stain poroly, e.g., incubated sections. The method stains well sections which cannot be stained with other rapid Nissl staining methods. |
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Keywords: | Nissl stain cryostat sections incubation in vitro autoradiography rapid staining |
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