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Determination of ergometrine maleate by fluorescence detection.
Authors:Zhengjun Gong  Yi Lv  Yuming Huang  Zhujun Zhang
Institution:School of Environmental Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. gongzhengjun@126.com
Abstract:A simple, accurate, sensitive and selective fluorescence analysis method for rapid determination of trace amounts of ergometrine maleate has been developed. The method is based on the fluorescence of ergometrine maleate. The calibration graph was linear in the range of 1 x 10(-4) to 0.2 microg/mL and the detection limit was 4 x 10(-5) microg/mL, with a relative standard deviation of 0.32% at 0.05 microg/mL (n = 11) ergometrine maleate. The influence of foreign compounds was tested. The proposed method was applied successfully to the determination of ergometrine maleate in pharmaceutical preparations and human plasma.
Keywords:
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