Study of rust resistance genes in wheat germplasm with DNA markers |
| |
| Authors: | Asma Haque Tayyaba Shaheen Tahsin Gulzar Mahmood ur Rahman Fatima Jalal Summera Sattar Beenish Ehsan Zafar Iqbal Muhammad Younas |
| |
| Affiliation: | 1.Department of Bioinformatics and Biotechnology, Government College University, Faisalabad;2.Department of Applied Chemistry, Government College University, Faisalabad;3.Agriculture Biotechnology Research Institute, AARI, Faisalabad |
| |
| Abstract: | Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogensand contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rustresistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversityand Nei and Li''s coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair GroupMethod of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereasminimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways toproduce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improvedcharacteristics. |
| |
| Keywords: | Wheat germplasm leaf rust stem rust stripe rust |
|
