Bilberry xyloglucan--novel building blocks containing beta-xylose within a complex structure |
| |
| Authors: | Hilz Hauke de Jong Laura E Kabel Mirjam A Verhoef René Schols Henk A Voragen Alphons G J |
| |
| Affiliation: | Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, PO Box 8129, 6700 EV Wageningen, The Netherlands. |
| |
| Abstract: | Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose-xylose (L) and fucose-galactose-xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, beta-xylose-alpha-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG. |
| |
| Keywords: | AIS, alcohol insoluble solids APTS, 8-aminopyrene-1,3,6-trisulfonate CASS, concentrated alkali soluble solids CE, capillary electrophoresis ESIMS, electrospray ionisation mass spectrometry HPAEC, high performance anion exchange chromatography LIFD, laser-induced fluorescence detection MALDI-TOFMS, matrix assisted laser desorption time of flight mass spectrometry PAD, pulse amperometric detection SPE, solid phase extraction XEG, xyloglucan specific endo-glucanase |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
