Cryopreservation of the milt of the northern pike |
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| Authors: | I Babiak J Glogowski M J Luczynski † D Kucharczyk ‡ M Luczynski § |
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| Institution: | Departments of Animal Biochemistry, Olsztyn University of Agriculture and Technology, 10–957 Olsztyn, Poland;Fisheries, Olsztyn University of Agriculture and Technology, 10–957 Olsztyn, Poland;Inland Fishery Institute, ul Oczapowskiego 10, 10–957 Olsztyn, Poland;Basic Fishery Sciences, Olsztyn University of Agriculture and Technology, 10–957 Olsztyn, Poland |
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| Abstract: | Seven published extenders, three thawing media and two thawing temperatures were tested in order to determine their suitability for cryopreservation of northern pike ( Esox lucius L.) sperm. Sperm motility during successive steps of cryopreservation was evaluated. Erdahl and Graham's extender with the addition of egg yolk proved to be the most efficient (maximum hatching rate of 74.5%) when semen was thawed in 120 m m NaCl solution warmed up to 30° C. No correlations between motility of sperm (diluted in extenders or diluted in extenders and then activated with thawing solution) and the subsequent hatching success were observed. The relationship between motility of thawed sperm and its fertilization ability was considerable, but correlation was not significant. Spermatozoa frozen in some extenders were frequently motile after thawing but they were not able to fertilize the eggs, this resulted in a poor hatching rate. Depending on the extender, the addition of yolk induced either positive or detrimental effects on fertilization success. |
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| Keywords: | fish Esox cryopreservation sperm fertilization motility |
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