The human transmembrane secretory component (poly-Ig receptor): molecular cloning,restriction fragment length polymorphism and chromosomal sublocalization |
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| Authors: | P Kraj?i K H Grzeschik A H M Geurts van Kessel B Olaisen P Brandtzaeg |
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| Institution: | (1) Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo The National Hospital, Rikshospitalet, N-0027, 1 Oslo, Norway;(2) Institut für Humangenetik der Universität, Bahnhofstrasse 7a, W-3550 Marburg, Germany;(3) Department of Human Genetics, University Hospital Nijmegen, NL-6500 HB Nijmegen, The Netherlands;(4) Institute for Forensic Medicine, University of Oslo, The National Hospital, Rikshospitalet, N-0027, 1 Oslo, Norway |
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| Abstract: | Summary The human transmembrane secretory component (SC) mediates glandular translocation of polymeric IgA and IgM into exocrine secretions. A 2898-bp cDNA clone, encoding the entire sequence of the human transmembrane SC, was isolated from a colonic adenocarcinoma cell line cDNA library. The deduced amino-acid sequence had a length of 764 residues and showed an overall similarity of 56% and 64% with the rabbit and rat counterpart, respectively. A restriction fragment length polymorphism (RFLP) was found with PvuII, revealing a two-alle RFLP with an autosomal codominant inheritance pattern and allele frequencies of 0.65 and 0.35. Southern blot analysis of human-rodent somatic hybrid panels, including hybrids with translocation chromosomes carrying different parts of chromosome 1, assigned the SC gene to 1q31-q42, thus confirming a previously reported provisional assignment. |
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