Subunit composition of rat liver glutathione S-transferases |
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| Authors: | C P Tu M J Weiss C C Reddy |
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| Affiliation: | 1. Department of Biochemistry, Microbiology, Molecular and Cell Biology The Pennsylvania State University, University Park, PA 16802 USA;2. Center for Air Environment Studies The Pennsylvania State University, University Park, PA 16802 USA |
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| Abstract: | The plasmid pGTR112 contains partial coding sequences for one of the rat liver glutathione S-transferase subunits. We have used immobilized pGTR112 DNA to select for complementary and homologous liver poly(A)-RNAs under conditions of increasing stringency for hybridization. Each fraction of selected poly(A)-RNAs was assayed by in vitro translation followed by immunoprecipitation. A total of four distinct polypeptides precipitated by antiserum against rat liver glutathione S-transferases were resolved by NaDodSO4 polyacrylamide gel electrophoresis. They are separated into two pairs according to the sequence homology of their poly(A)-RNAs with the pGTR112 DNA. Purified rat liver glutathione S-transferases can be resolved on gradient NaDodSO4 polyacrylamide gels into four polypeptides. There should be ten isozymes of different binary combinations from four distinct subunits for the rat liver glutathione S-transferases. |
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| Keywords: | To whom correspondence should be addressed. |
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