Analysis of 2',5'-oligoadenylates in cells and tissues |
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Authors: | T R Reid C L Hersh I M Kerr G R Stark |
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Institution: | 1. Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 USA;2. Imperial Cancer Research Fund, Lincoln''s Inn Fields, London WC2A 3PX, England |
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Abstract: | Complex mixtures of 2',5'-oligoadenylates are formed in cells and tissues under several different circumstances, and methods for analyzing such mixtures are reviewed. Separation is achieved by high-performance liquid chromatography and quantitation by competition-binding assays, using three different types of antibodies or a specific binding protein, or by functional assay, using preparations of an endonuclease specifically activated by some of the 2',5'-oligoadenylates. Representative results from three different biological systems are presented. The function of 2',5'-oligoadenylates as activators of intracellular RNA degradation is discussed, along with the possibility that these compounds may serve as signals for other intracellular regulatory processes. |
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Keywords: | To whom correspondence should be addressed at the Imperial Cancer Research Fund |
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