前列腺素E在骨髓基质调节造血中的作用

小鼠骨髓细胞体外液体培养,形成基质细胞层(SL),能影响在其上培养的粒系祖细胞(CFU-GM)增殖。培养1周,SL明显抑制CFU-GM,约为对照的50％;2周,SL之抑制减弱,3周,SL则促进CFU-GM,约达对照140％。在CFU-GM培养体系中加入消炎痛(1×10~(-7)mol/L),却使1周SL上CFU-GM增加,2周SL上者增加更显著,第3周者则无明显改变。在CFU-GM培养体系中加入PGE_1(1×10~(-8)mol/L),各周SL上CFU-GM均大为减少。若同时再加消炎痛(2×10~(-7)mol/L),基质层上CFU-GM,1周者上升到约为对照42.6％,2周者则接近对照。而3周者则用消炎痛1×10~(-7)mol/L,即可使CFU-GM产率恢复。说明SL培养1周,能生成一定量PGE,2周时PGE生成减少,3周则几乎为零。故SL影响CFUGM,至少部分地以PGE为中介。

ROLE OF PROSTAGLANDIN E IN MODULATION OF GRANULOPOIESIS BY MARROW STROMA

The stroma layer (SL) cultured from mouse bone marrow cells for one week, had an inhibitory effect on the granulocyte-macrophage progenitor cells (CFU-GM). The inhibitory effect decreased in the 2nd week SL, while the 3rd week SL promoted CFU-GM growth greatly. When indomethacin (1 x 10(-7) mol/L) was added into the CFU-GM culture system, the score of CFU-GM on the 1st week SL was raised, and on the 2nd week SL was further increased significantly, but it leveled off on the 3rd week SL. The addition of exogenous 10(-8) mol/L PGF1 suppressed the CFU-GM growth on all the 1st to 3rd week SLs. When 1 x 10(-8) mol/L PGE1 was added with 2 x 10(-7) mol/L indomethacin, 1st week SL-CFU-GM increased to 42.61%, 2nd week SL-CFU-GM nearly to 100% of the control. For the 3rd week SL-CFG-GM, 1 x 10(-7) mol/L indomethacin was enough to reverse the inhibition induced by exogenous 1 x 10(-8) mol/L PGE1. It is suggested that definite amount of PGE was produced from cells in the 1st week SL. The secretion of PGE from SL was reduced during 2nd week, and almost stopped in the 3rd week SL. The results indicate that the modulation of granulopoiesis by marrow stroma is, at least partly, mediated by PGE.