| 影响离体大鼠心肌细胞和蛋白重组脂质体Na^＋—Ca^2＋交换的因素 |
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| 引用本文： | 李兆萍,唐朝枢.影响离体大鼠心肌细胞和蛋白重组脂质体Na^＋—Ca^2＋交换的因素[J].生理学报,1989,41(5):470-475. |
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| 作者姓名： | 李兆萍 唐朝枢 |
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| 作者单位： | 北京医科大学病理生理教研室
(李兆萍，唐朝枢)，北京医科大学病理生理教研室(苏静怡) |
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| 摘 要： | 本工作在离体成年大鼠心室肌细胞和狗心肌肌膜Na~ -Ca~(2 )交换蛋白重组脂质体上,发现预先用哇巴因孵育细胞使细胞内Na~ 浓度升高或降低细胞外Na~ 浓度均使细胞及脂质体的Na~ -Ca~(2 )交换增加。Mn~(2 )对细胞和脂质体的Na~ -Ca~(2 )交换呈剂量依赖性的抑制作用;异搏定则无明显影响;花生四烯酸对Na~ -Ca~(2 )交换有激活作用;过氧化氢引起膜脂质过氧化后,显著促进脂质体的Na~ -Ca~(2 )交换,并呈时间和剂量依赖性。
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| 关 键 词： | 心肌细胞 脂质体 Na~ -Ca~(2 )交换 哇巴因 Mn~(2 ) 异搏定 花生四烯酸 过氧化氢 |
FACTORS AFFECT Na~ -Ca~(2 ) EXCHANGE IN RAT'S CARDIOMYOCYTES AND PROTEIN-RECONSTITUTED LIPOSOMES |
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| LI ZHAO-PING,TANG CHAO-SHU AND SU JING-YI.FACTORS AFFECT Na~ -Ca~(2 ) EXCHANGE IN RAT'S CARDIOMYOCYTES AND PROTEIN-RECONSTITUTED LIPOSOMES[J].Acta Physiologica Sinica,1989,41(5):470-475. |
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| Authors: | LI ZHAO-PING TANG CHAO-SHU AND SU JING-YI |
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| Abstract: | Rat's Ca-resistant ventricular myocytes and reconstituted liposomes of canine cardiac sarcolemma were used in analysing factors affecting Na~ -Ca~(2 ) exchange system. Decrease in extracellular(extravesicular) Na~ or increase in intracellular Na~ by inhibition of the Na~ -K~ -ATPase with ouabain stimulated the Na~ -Ca~(2 ) exchange. Mn~(2 ), an antagonist of Na~ -Ca~(2 ) exchange, dose-dependently inhibited the Na~ -dependent Ca~(2 ) uptake. However, verapamil had no effect on it. Arachidonic acid and lipid peroxides caused by incubation withH_O_2 significantly stimulated Na~ -Ca~(2 ) exchange in a dose-dependent fashin. |
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| Keywords: | cardiomyocyte liposome Na~ -Ca~(2 ) exchange ouabain Mn~(2 ) verapa mil arachidonic acid H_2O_2 |
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