酪氨酸对人离体滋养层细胞孕酮与hCG分泌的影响

本文观察三种剂量(2×10~(-5)mol/L,2×10~(-4)mol/L和2×10~(-3)mol/L)的酪氮酸对离体培养的滋养层细胞孕酮及hCG分泌的影响,并对其抑制效应的机理作了初步探讨。实验结果表明,三种剂量的酪氨酸均可抑制滋养层细胞孕酮分泌(P<0.01),但是,在孕酮分泌受酪氨酸抑制的同时,未见对hCG分泌发生影响(P>0.05),进一步观察了酪氨酸对滋养层细胞3β-羟甾脱氢酶活性的影响,结果表明,酪氨酸能显著抑制3β-羟甾脱氢酶活性,提示酪氨酸对滋养层细胞孕酮生成的抑制作用与抑制3β-羟甾脱氢酶活性有关。

EFFECT OF TYROSINE ON PROGESTERONE AND hCG PRODUCTION BY HUMAN TROPHOBLAST CELLS IN VITRO

The purpose of the present study was to investigate the effect of tyrosine on progesterone and hCG production by human placental cells in vitro. The results showed that the three concentrations (0.02 mmol/L, 0.2 mmol/L and 2mmol/L) of tyrosine inhibited progesterone secretion by trophoblast cells in vitro. While progesterone secretion was suppressed by tyrosine, hCG secretion was unchanged. Thus, it seems unlikely that the suppression of progesterone production by tyrosine was due to a decrease in hCG production. It was further demonstrated that tyrosine inhibited 3 beta-HSD activity of trophoblast cells, indicating that the progesterone production inhibition by them might be due to the decreased 3 beta-HSD activity of trophoblast cells.