Embryonic chicken gizzard: smooth muscle and non-muscle myosin isoforms |
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Authors: | Elke R. Paul Anna-Luise Christian Renate Franke Ute Gröschel-Stewart |
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Affiliation: | (1) Institut für Zoologie, Technische Hochschule Darmstadt, Schnittspahnstrasse 10, D-64287 Darmstadt, Germany |
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Abstract: | Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independently at the luminal side close to the epithelial layer, and the presumptive capillaries invade the developing muscle in a luminal to serosal direction. The smooth muscle and non-muscle myosin heavy chains in this avian system cannot be separated by SDS polyacrylamide gel electrophoresis and do not show isoform specificity in immunoblotting, unlike the system found in mammals. Only two myosin heavy chains with Mr of 200 and 196 kDa were separable and considerable immunological cross-reactivity was found between the denatured myosin isoform heavy chains. |
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Keywords: | Gizzard Development, ontogenetic Muscle smooth Capillaries Immunohistochemistry Myosin Domestic fowl |
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