| 猪生长激素基因表达质粒的构建及其转基因动物研究 |
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| 引用本文： | 陈清轩,曹颉,宋德秀,陈永福,魏庆信.猪生长激素基因表达质粒的构建及其转基因动物研究[J].中国生物化学与分子生物学报,1997,13(5):540-545. |
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| 作者姓名： | 陈清轩 曹颉 宋德秀 陈永福 魏庆信 |
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| 作者单位： | 中国科学院发育生物学研究所，北京农业大学生物学院，湖北省农科院畜牧兽医研究所 |
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| 摘 要： | 将猪生长激素基因（ＰＧＨ）克隆到质粒ｐＵＣ１９上，经酶切分析，确定其酶切图谱．把ＰＧＨ转录起始位点以前的序列切掉，换上羊ＭＴ－１ａ基因的启动子，构建成可以调控的表达载体ｐＳＭＴＰＧＨ，用于转基因动物的研究．采用微注射法将线状ｐＳＭＴＰＧＨ导入猪、鼠和金鱼的受精卵中，得到了相应的转基因动物．对这些动物鉴定分析表明，外源基因整合率因动物不同而异，但该基因在这３种动物中的整合率均在９％以上，其生长速度都高于对照组．
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| 关 键 词： | 猪生长激素基因 表达载体pSMTPGH 转基因动物 |
| 收稿时间： | 1997-10-20 |
The Construction of Porcine Growth Hormone Gene Expressive |
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| Plasmid and the Studies of Transgenic Animals,Chen Qing Xuan Cao Jie Song De Xiu.The Construction of Porcine Growth Hormone Gene Expressive[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(5):540-545. |
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| Authors: | Plasmid and the Studies of Transgenic Animals Chen Qing Xuan Cao Jie Song De Xiu |
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| Institution: | (Institute of Developmental Biology,Academia Sinica,Beijing 100080) Chen Yong Fu (Beijing Agricultural University,Insititute of Biology,Beijing 100094) Wei Qin |
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| Abstract: | The expressive plasmid pSMTPGH was constructed with porcine growth hormone gene (PGH) and sheep metallothionein promoter (SMT).The linear fusion gene constructs were microinjected into zygots of mice,pigs and gold fishes separately.Three different transgenic animals were produced,in which the foreign gene constructs were intergrated at the rate of over 9 0%.The average growth rate of the transgenic animals are higher than control animals (normal animals).These results demonstrate that the production of stable lines of transgenic farm aninals with enhanced growth performances is feasible. |
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| Keywords: | Porcine growth hormone gene plasmid pSMTPGH Transgenic animals |
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