Oxidative Damage of Lysozyme and Human Serum Albumin and Their Mixtures. A Comparison of Photosensitized and Peroxyl Radical Promoted Processes |
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Authors: | Andrea Arenas Rodrigo Vasquez Camilo López-Alarcón Eduardo Lissi Eduardo Silva |
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Institution: | Departamento de Química Física, Pontificia Universidad Católica de Chile, Santiago. |
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Abstract: | Oxidative modifications of lysozyme (Lyso) and human serum albumin (HSA) mediated by photoinduced processes and peroxyl radicals
were studied. Both oxidative conditions were applied to the separate proteins and their mixtures. Dimerization and fragmentation
of the proteins do not correlate with the formation of carbonyls or peroxides, implying that evaluation of these changes is
not an index of the overall oxidative modification of a protein. The results obtained also show that the hypothesis that the
electrostatic interactions of Lyso and HSA could facilitate the formation of Lyso-HSA dimers in the presence of a source of
reactive oxygen species was verified in both ROS-producing systems. |
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