| 我国戊型肝炎病毒基因组cDNA全序列测定及分析 |
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| 引用本文： | 毕胜利 曹学义. 我国戊型肝炎病毒基因组cDNA全序列测定及分析[J]. 病毒学报, 1992, 8(3): 271-279 |
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| 作者姓名： | 毕胜利 曹学义 |
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| 作者单位： | 中国预防医学科学院病毒学研究所(毕胜利，刘崇柏，赵洪兰)，新疆维吾尔自治区卫生防疫站(曹学义)，美国疾病控制中心肝炎实验室(H.S.Margolis) |
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| 摘 要： |
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| 关 键 词： | 聚合酶链反应 戊肝病毒 |
MOLECULAR CLONING AND SEQUENCING OF THE WHOLE CHINESE HEPATITIS E VIRUS GENOME |
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| Bi Shengli Liu Chongbai Cao Xiuyi Zhao HonglanH.S.Margolis. MOLECULAR CLONING AND SEQUENCING OF THE WHOLE CHINESE HEPATITIS E VIRUS GENOME[J]. Chinese journal of virology, 1992, 8(3): 271-279 |
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| Authors: | Bi Shengli Liu Chongbai Cao Xiuyi Zhao HonglanH.S.Margolis |
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| Affiliation: | Bi Shengli Liu Chongbai Cao Xiuyi Zhao HonglanH.S.Margolis1,Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 2,Sanitary and Anti-epidemic Station,Xinjiang Autonomous 3.Center for Diseases Control USA |
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| Abstract: | A solid phase antibody capture method has been developed to concen trat and purify the HEV particles from fecal samples. The samples were collected from an acute phase HEV patient during the epidemic of 1986 1987, in Xinjiang Ugur Autonomous Region, China.A total of 7.4kb of full-length HEV cDNA clone was generated and sequenced by Dideoxy chain termination method. There are 94% homology in nucleotides when compared with the genome of HEV cloned originally from a Burma HEV strain. The constituent ratio of the Chinese HEV genome was composed of 17.1% adenine, 24.9% thymine, 32.1% cytosine, and 25.9% guanine. The C + G content was 58%. |
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| Keywords: | Hepatitis E virus Polymerase chain reaction |
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