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Construction and characterization of a porcine P1-derived artificial chromosome (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six type I and type II loci |
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| Authors: | Hiam K Al-Bayati Sonja Duscher Sonja Kollers Günther Rettenberger Ruedi Fries Bertram Brenig |
| Institution: | Institute of Veterinary Medicine, University of G?ttingen, Groner Landstrasse 2 37073 Goettingen, Germany, DE Institute of Animal Breeding, Technical University of Munich/Weihenstephan, Germany, DE Medical Laboratory for Human Genetics Dr. Mehnert, Neu-Ulm, Germany, DE |
| Abstract: | A porcine P1-derived artificial chromosome (PAC) library of a male German Landrace pig was constructed in pCYPAC2. In total 90,240 clones were generated and individually transferred into microtiter plates. An average insert size of 119.1 kb was determined by analyzing 150 randomly selected PAC clones by pulsed field electrophoresis, yielding approximately 3.2 genome equivalents. The stability of nine clones was followed through 110 generations showing no reduction of the insert size. The probability of identifying a specific chromosomal region within the library was tested by screening for the presence of seven type I and five type II loci. The analysis showed that most loci (10/12) were present in the library at least twice. To determine the percentage of chimerism, six clones were analyzed by fluorescence in situ hybridization (FISH) on metaphase chromosomes. We assign one type I locus (Triadin) and three type II loci (SW855, S0300, SW1129). Received: 24 November 1998 / Accepted: 1 February 1999 |
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