Isolation of 15 single nucleotide polymorphisms from coastal steelhead, Oncorhynchus mykiss (Salmonidae) |
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Authors: | Aguilar Andres Garza John Carlos |
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Affiliation: | School of Natural Sciences, University of California, Merced, PO Box 2039, Merced, CA 95340, USA, NOAA Southwest Fisheries Science Center, 110 Shaffer Road, Santa Cruz, CA 95060, USA, Institute of Marine Sciences, University of California, Santa Cruz, 110 Shaffer Road, Santa Cruz, CA 95060, USA. |
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Abstract: | We describe 15 single nucleotide polymorphisms (SNPs) isolated in coastal California populations of steelhead (Oncorhynchus mykiss). SNP loci were developed using a 'gene-targeted' approach, which involved the development of primers from functional genes in O. mykiss that were deposited in GenBank or in the published literature. These markers show a wide range of variability in three coastal steelhead populations, and will be useful in population genetic studies and in pedigree reconstruction. Potential applications include evaluation of population structure, introgression between native and hatchery trout, and evaluating reproductive success. |
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Keywords: | linkage Oncorhynchus mykiss single nucleotide polymorphisms SNPs steelhead |
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