A microarray system for Y chromosomal and mitochondrial single nucleotide polymorphism analysis in chimpanzee populations |
| |
Authors: | Andrés Olga Rönn Ann-Charlotte Bonhomme Maxime Kellermann Thomas Crouau-Roy Brigitte Doxiadis Gaby Verschoor Ernst J Goossens Benoît Domingo-Roura Xavier Bruford Michael W Bosch Montserrat Syvänen Ann-Christine |
| |
Affiliation: | Genètica de la Conservació Animal, Institut de Recerca i Tecnologia Agroalimentàries, Carretera de Cabrils Km2, 08348 Cabrils, Spain Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Carrer Doctor Aiguader 80, 08003 Barcelona, Spain Molecular Medicine, Department of Medical Sciences, Uppsala University, University Hospital, Entr. 70, 3rd floor, Res. Department 2, 75185 Uppsala, Sweden Evolution et Diversité Biologique UMR 5174 CNRS, Université Paul Sabatier Toulouse, 118 route de Narbonne, Bat IV R3 (b2), 31062 Toulouse cedex 4, France Institut für Immungenetik, Charité-Universit?tsmedizin Berlin, Campus Virchow-Klinikum, Humboldt Universit?t zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands Cardiff School of Biosciences, Cardiff University, PO Box 915 Cathays Park, Cardiff CF10 3TL, UK. |
| |
Abstract: | Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms (SNP) are biallelic markers that are widely used in human molecular studies and can be implemented in efficient microarray systems. This technology offers the potential of robust, multiplexed SNP genotyping at low reagent cost in other organisms than humans, but it is not commonly used yet in wild population studies. Here, we describe the characterization of new SNPs in Y-chromosomal intronic regions in chimpanzees and also identify SNPs from mitochondrial genes, with the aim of developing a microarray system that permits the simultaneous study of both paternal and maternal lineages. Our system consists of 42 SNPs for the Y chromosome and 45 SNPs for the mitochondrial genome. We demonstrate the applicability of this microarray in a captive population where genotypes accurately reflected its large pedigree. Two wild-living populations were also analysed and the results show that the microarray will be a useful tool alongside microsatellite markers, since it supplies complementary information about population structure and ecology. SNP genotyping using microarray technology, therefore, is a promising approach and may become an essential tool in conservation genetics to help in the management and study of captive and wild-living populations. Moreover, microarrays that combine SNPs from different genomic regions could replace microsatellite typing in the future. |
| |
Keywords: | chimpanzee conservation genetics microarray minisequencing population genetics sex‐linked SNPs |
本文献已被 PubMed 等数据库收录! |
|