Isolation and characterization of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul (Phyllastrephus flavostriatus) and cross-species amplification in four closely related taxa |
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| Authors: | Lokugalappatti L G Sampath Feldheim Kevin A Sellas Anna B Bowie Rauri C K |
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| Affiliation: | 1. DST/NRF Centre of Excellence at the Percy FitzPatrick Institute, Department of Botany and Zoology, Stellenbosch University, Private BagXI, Matieland 7602, South Africa,;2. Museum of Vertebrate Zoology and Department of Integrative Biology, 3101 Valley Life Science Building, University of California, Berkeley, CA 3. 94720, USA,;4. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 5. S. Lake Shore Drive, Chicago, IL 6. 60605, USA |
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| Abstract: | We describe the isolation of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul using an enrichment protocol. All loci were highly variable with the number of alleles ranging from 8 to 13, and observed heterozygosity ranging from 0.652 to 0.870. All loci were in Hardy-Weinberg equilibrium; however, loci Pfl12 and Pfl54 showed significant linkage disequilibrium. All 10 loci successfully amplified and were polymorphic in at least one of four related Phyllastrephus species. These loci should prove to be widely applicable to studies of phylogeography, hybridization and paternity in African greenbuls. |
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| Keywords: | Africa Aves bulbuls greenbuls Pycnonotidae tetranucleotides |
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