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Isolation of 10 tetranucleotide microsatellite loci in the blackcap (Sylvia atricapilla) |
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| Authors: | Segelbacher Gernot Rolshausen Gregor Weis-Dootz Tanja Serrano David Schaefer H Martin |
| Institution: | 1. Department of Wildlife Ecology and Management, University Freiburg, Tennenbacher Str. 4, D‐79106 Freiburg, Germany,;2. Department of Evolutionary Biology and Animal Ecology, Faculty of Biology, University Freiburg, Hauptstrasse 1, D‐79108 Freiburg, Germany,;3. Estación Biológica de Do?ana (CSIC), Pabellón del Perú, Avda. Maria Luisa s/n, 41013 Sevilla, Spain |
| Abstract: | We isolated 10 polymorphic microsatellite DNA loci from the blackcap (Sylvia atricapilla) and optimized them for future studies of population differentiation in populations with different migration strategies in southwestern Germany. The loci were screened for polymorphism using 178 individuals from two populations in Germany and Spain. The primers amplified highly variable loci characterized by two to 19 alleles per locus and their observed and expected heterozygosities range from 0.47 to 0.81 and from 0.50 to 0.91, respectively. |
| Keywords: | primer Sylvia atricapilla tetranucleotide microsatellites |
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