Isolation and characterization of microsatellite loci in the deep-sea marine fish, the roundnose grenadier (Coryphaenoides rupestris) |
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| Authors: | Knutsen Halvor LE Goff-Vitry Marie Fiani David Hoelzel A Rus |
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| Institution: | 1. Fl?devigen Marine Research Station, Department of Coastal Zone, Institute of Marine Research, N‐4817 His, Norway,;2. School of Ocean & Earth Science, University of Southampton, Southampton Oceanography Centre, European Way, Southampton SO14 3ZH, UK;3. Present address: School of Biosciences 2, Cardiff University, Cardiff CF10 3US, UK;4. School of Biological and Biomedical Sciences, Durham University, South Road, Durham, DH1 3LE UK, |
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| Abstract: | We developed polymerase chain reaction primers for eight dinucleotide microsatellite loci in the marine deep sea fish, roundnose grenadier (Coryphaenoides rupestris). All markers were obtained from a partial genomic DNA library, and characterized in 90 unrelated individuals from one putative population sampled on the Mid-Atlantic Ridge. The number of alleles ranged from two to 61 with an average of 21 per locus. The observed heterozygosity levels ranged from 0.301 to 0.987 with an average of 0.672. Several of the markers amplified multiple alleles from either the Atlantic cod (Gadus morhua) or the deep-sea fish roughhead grenadier (Macrourus berglax). |
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| Keywords: | Coryphaenoides rupestris genetic diversity microsatellite primers polymorphic |
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