Development of nine microsatellite markers for Pomacentrus amboinensis |
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Authors: | Jones David B Jerry Dean R McCormick Mark I Bay Line K |
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Institution: | School of Marine and Tropical Biology, James Cook University, Townsville, Qld 4811, Australia, ARC Centre of Excellence for Coral Reef Studies, Townsville, Qld 4811, Australia. |
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Abstract: | The relatively long pelagic larval duration of Pomacentrus amboinensis, a tropical fish, suggests the potential for long-distance dispersal; however, several nongenetic studies have found substantial self-recruitment at one location. To analyse patterns of connectivity of this species, primers for nine independent microsatellite loci were developed for P. amboinensis using a magnetic bead enrichment protocol. Twenty individuals from one location were analysed and observed heterozygosities ranged from 0.7 to 0.95. Eight of nine loci were in Hardy-Weinberg equilibrium and no evidence of linkage or null alleles were found. |
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Keywords: | connectivity microsatellite primers Pomacentrus amboinensis tropical fish |
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