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Characterization of microsatellite loci in the lesser dawn bat (Eonycteris spelaea) |
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| Authors: | Shao W W Hua P Y Zhou S Y Zhang S Y Chen J P |
| Institution: | Guangdong Entomological Institute, Guangzhou 510260, China, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, School of Life Science, Guangxi Normal University, Guilin 541004, China, School of Life Science, East China Normal University, Shanghai 200062, China. |
| Abstract: | We constructed a genomic DNA library enriched for CA repeat motifs in Eonycteris spelaea. Nine microsatellite loci were isolated and tested on a population of 39 samples from Yunnan Province, China. These nine loci had three to 22 alleles per locus. Observed and expected heterozygosity values ranged from 0.079 to 0.963 and from 0.078 to 0.959. Two loci revealed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was found between loci pairs. These microsatellites can be a powerful molecular tool for population-level studies of E. spelaea. |
| Keywords: | Eonycteris spelaea microsatellite loci polymorphism |
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