Non-invasive genetic identification of small mammal species using real-time polymerase chain reaction |
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Authors: | Moran S Turner P D O'Reilly C |
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Affiliation: | Department of Chemical and Life Sciences, Waterford Institute of Technology, Cork Road, Waterford, Ireland. |
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Abstract: | DNA identification of non-invasive samples is a potentially useful tool for monitoring small mammal species. Here we describe a novel method for identifying five small mammal species: wood mouse, bank vole, common shrew, pygmy shrew and water shrew. Species-specific real-time polymerase chain reaction primers were designed to amplify fragments of the mitochondrial cytochrome b gene from hair and scat samples. We also amplified nuclear DNA from scats, demonstrating their potential as a source of DNA for population genetic studies. |
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Keywords: | cytochrome b non‐invasive genetic sampling real‐time PCR small mammals species identification |
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