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New polymorphic microsatellite markers for the limpet Patella rustica and cross-priming testing in four Patella species |
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| Authors: | Pérez M Llavona A Presa P Ribeiro P Hawkins S J Santos A M Alexandrino P |
| Institution: | 1. CIBIO, Centro de Investiga??o em Biodiversidade e Recursos Genéticos, Campus Agrário de Vair?o, 4485‐661 Vair?o, Portugal,;2. Department of Biochemistry, Genetics and Immunology, Faculty of Marine Sciences, University of Vigo, 36310 Vigo, Spain,;3. ECIMAT. Estación de Ciencias Marinas de Toralla, Universidad de Vigo, 36331 Vigo, Spain,;4. University of Southampton, Southampton S09 5NH, UK;5. Marine Biological Association, Plymouth PL1 2PB, UK;6. Departamento de Zoologia‐Antropologia, Faculdade de Ciências da Universidade do Porto, 4099‐002 Porto, Portugal, |
| Abstract: | Eighteen microsatellite markers have been characterized from the Patella rustica genome. An average of 10 alleles per locus and an expected heterozygosity ranging from 0.054 to 0.937 were observed in a sample of 32 wild individuals from Viana do Castelo, Portugal. Distinct cross-priming amplification rates were recovered on four additional Patella species. Three monomorphic P. rustica loci were polymorphic in other Patella species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine population studies in the genus Patella. |
| Keywords: | cross‐priming limpets microsatellites Patella rustica |
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