| 利用FPLC提纯抗苯丙氨酸羟化酶抗体的Fab及其轻链和重链的分离 |
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| 引用本文： | 徐秀璋,F.J.Morgan.利用FPLC提纯抗苯丙氨酸羟化酶抗体的Fab及其轻链和重链的分离[J].中国生物化学与分子生物学报,1990,6(5):394-399. |
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| 作者姓名： | 徐秀璋 F.J.Morgan |
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| 作者单位： | 中国科学院生物物理所 北京
(徐秀璋)，墨尔本St.Vincent医学研究所 澳大利亚(F.J.Morgan) |
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| 摘 要： | IgGPH_7经木瓜蛋白酶消解产生Fab和Fc,在FPLC仪上,分别用MonoQ,Superose_(12),MonoS柱提纯Fab。提纯的Fab经还原和烷基化后,再用琥珀酸酐修饰,然后用Mono Q柱分离得到轻链和重链。最后通过SDS凝胶电泳和N端顺序测定,以鉴定它的纯度和轻、重链。
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| 关 键 词： | 纯化 FPLC Fab 轻链 重链 |
| 收稿时间： | 1990-10-20 |
Preparation of the Fab Fragment of an Anti-Phenylalanine Hydroxylase Antibody and Separation of Light Chain and Heavy Chain From the Fab Only Using FPLC |
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| Xu,Xiuzhaug F.J.Morgan.Preparation of the Fab Fragment of an Anti-Phenylalanine Hydroxylase Antibody and Separation of Light Chain and Heavy Chain From the Fab Only Using FPLC[J].Chinese Journal of Biochemistry and Molecular Biology,1990,6(5):394-399. |
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| Authors: | Xu Xiuzhaug FJMorgan |
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| Institution: | (Institute of Biophysics Academia Sinica, Beijing)(St. Vincent's Institute of Medical Research, Melbourne Australia |
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| Abstract: | Digestion of IgGPH7 by the enzyme papain produced Fab fragments and Fc fragments.The Fab were purified by Mono Q column, Superose 12 column and Mono S column respectively on the FPLC equipment. Then the Light Chain and the Heavy Chain from the Fab were separated by Mono Q column after purified Fab were reduced, alkylated and modified. Separated Light Chain and Heavy Chain of Fab were identified by SDS-PAGE and determination of the N-terminal amino acid sequence . |
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| Keywords: | Purification FPLC Fab Light Chain Heavy Chain |
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