Expression of four types of human tyrosine hydroxylase in COS cells |
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Authors: | K Kobayashi K Kiuchi A Ishii N Kaneda Y Kurosawa K Fujita T Nagatsu |
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Affiliation: | Department of Biochemistry, Nagoya University School of Medicine, Japan. |
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Abstract: | Alternative splicing from a single gene produces four kinds of human tyrosine hydroxylase (types 1-4), which have structural diversity only in the N-terminal region. We attempted expression of the type 1-4 enzymes in COS cells and performed kinetic analyses. All had enzymatic activities. The Km values of the four types for L-tyrosine and 6-methyl-5,6,7,8-tetrahydropteridine were similar, although their relative homospecific activities were clearly different. The type 1 enzyme displayed the highest activity. |
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