酪酸梭菌与双歧杆菌对肠道致病菌的体外生物拮抗作用

目的：探讨酪酸梭菌和婴儿型双歧杆菌对某些肠道致病菌的拮抗作用，方法：将酪酸梭菌和婴和型双歧杆菌单株菌及两株菌混合后分别与几株肠道致病菌混合接种于GAM液体培养其中进行厌氧培养，通过平板菌落计数法计算肠道致病菌的菌数，结果：经混合培养后肠道致病菌的菌数明显下降，结论：酪酸梭菌LCL166株和婴儿型双歧杆菌LCL172株在体外能明显抑制几株肠道致病菌的生长繁殖。

STUDIES ON THE ANTAGONISM OF CLOSTRIDIUM BUTYRICUM LCL166 STRAIN AND BIFIDOBACTERIUM LCL172 STRAIN AGAINST SOME ENTERPATHOGENS IN VITRO

The pure culture of Clostridium butyricum LCL166 strain alone ,B ifidobacterium infantis LCL172 (Bif,infantis LCL172)strain alone and mixed cultu res of C butyricum LCL166 and Bif infantis LCL 172 was mixed in vitr o with that o f E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 propot ionally in GA M culture medium respectively During anaerobic incubation,quantities of each en t erpathogens in the mixed cultures were investigate As a result,the amounts of E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 in mixed cult ures of C butyricum LCL 166 and Bif infantis LCL172 were significantly lower than t heir pure culture alone respectively