Isolierung von Auxinen aus dem Chromatin regenerierender Erbsenkeimlinge

Summary Etiolated pea epicotyls were treated with IAA, NAA or 2,4-D for 48 hours. From the chromatin (purified by gel-chromatography) of the treated seedlings growth substances could be isolated which significantly promote growth of Avena coleoptiles. The isolated growth substances showed the same RF as the applied auxins. When the seedlings were treated for 48 hours either with auxin or with water only the extract from the auxin treated plants promoted Avena growth significantly. The ether extract from the auxin treated seedlings still had growth promoting effect when the seedlings were treated 24 hours with auxin and then 24 hours with water to remove most of the free auxin in the cells and in the cell walls.