首页 | 本学科首页   官方微博 | 高级检索  
   检索      


A two-step enzymatic synthesis of dipeptides
Authors:Schwarz A  Wandrey C  Steinke D  Kula M R
Institution:Institute of Biotechnology, Research Center Jülich, D-5170 Jülich, Germany.
Abstract:A simple system is introduced to produce dipeptides continuously by enzyme catalyzed condensation of amino acid esters and amino acid amides. Synthesis of N-terminal free dipeptide-amides is achieved by means of carboxypeptidase Y. The peptide-amide is deamidated utilizing a newly isolated peptide-amide is deamidated utilizing a newly isolated peptide-amidase. Separation of substrates and products is accomplished by anion-exchange chromatography. Modeling of the reactions shows that the two reactions have to be carried out in a cascade of two reactors in order to prevent hydrolysis of the peptide by the carboxypeptidase. Continuous production of Kyotorphin (H-TyrArg-OH) with a space-time yield of 257 g/L . d shows the feasibility of this concept.
Keywords:enzymatic synthesis  continuous production  anion-exchange  peptide-amidase
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号