A two-step enzymatic synthesis of dipeptides |
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Authors: | Schwarz A Wandrey C Steinke D Kula M R |
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Institution: | Institute of Biotechnology, Research Center Jülich, D-5170 Jülich, Germany. |
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Abstract: | A simple system is introduced to produce dipeptides continuously by enzyme catalyzed condensation of amino acid esters and amino acid amides. Synthesis of N-terminal free dipeptide-amides is achieved by means of carboxypeptidase Y. The peptide-amide is deamidated utilizing a newly isolated peptide-amide is deamidated utilizing a newly isolated peptide-amidase. Separation of substrates and products is accomplished by anion-exchange chromatography. Modeling of the reactions shows that the two reactions have to be carried out in a cascade of two reactors in order to prevent hydrolysis of the peptide by the carboxypeptidase. Continuous production of Kyotorphin (H-TyrArg-OH) with a space-time yield of 257 g/L . d shows the feasibility of this concept. |
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Keywords: | enzymatic synthesis continuous production anion-exchange peptide-amidase |
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