| 补料方式对酵母菌生产谷胱甘肽的影响 |
| |
| 引用本文： | 童群义,王国成,堵国成,陈坚.补料方式对酵母菌生产谷胱甘肽的影响[J].工业微生物,2003,33(1):19-22. |
| |
| 作者姓名： | 童群义 王国成 堵国成 陈坚 |
| |
| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214036 |
| |
| 基金项目： | 中国博士后科学基金 (第 2 7批 )资助项目 |
| |
| 摘 要： | 比较了酵母菌发酵生产谷胱甘肽（GSH）的几种补料分批培养方式。实验发现补料可以明显地促进酵母菌的生长和谷胱甘肽的合成，同时还发现不同的补料方式对发酵液中的菌体浓度和GSH浓度有不同的影响。采用指数流加方式可获得极高的菌体浓度，但菌体中的GSH浓度较低；而采用恒－pH补料分批培养既可以达到较高菌体浓度，菌体中又含有较高的GSH含量，因此，其总的GSH产量最高，可达到977.8mg/L。
|
| 关 键 词： | 补料分批培养 酵母菌 谷胱甘肽 生产 |
Effects of feeding method on production of glutathione by Saccharomyces cerevisiae |
| |
| TONG Qun-yi,WANG Guo-cheng,DU Guo-cheng,CHEN Jian.Effects of feeding method on production of glutathione by Saccharomyces cerevisiae[J].Industrial Microbiology,2003,33(1):19-22. |
| |
| Authors: | TONG Qun-yi WANG Guo-cheng DU Guo-cheng CHEN Jian |
| |
| Abstract: | Several feeding fed-batch culture methods for the production of glutathione (GSH) by Saccharomyces cerevisiae were compared. The results showed that the feeding could obviously promote the growth of S.cerevisiae and the synthesis of glutathione, and different feeding methods had different influences on the biomass and GSH in the broth. The highest biomass could obtain by exponential feeding, but the concentration of GSH was lower; higher biomass and higher GSH could be obtained by pH-stat fed-batch culture, the total concentration of GSH (977.8mg/L) was highest. |
| |
| Keywords: | fed-batch culture Saccharomyces cerevisiae glutathione |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
