环境毒物对金黄色葡萄球菌毒性影响的研究

采用毒物在营养琼脂中垂直扩散方法，通过测定毒物在营养球脂中抑制金黄色葡萄球菌生长产生蓝色抑菌带的长度，研究Hg、Cr^6 、Pb、CN^—、As、NO2^—、F^—及苯酚对金黄色葡萄球菌的毒性影响。结果表明：受试毒物的浓度与抑菌带有相关性，相关系数具显著意义；对毒物的敏感性为Cr^6 ＞Hg＞As＞CN^—＞Pb＞NO2^—＞苯酚＞F—；多种毒物共同作用其毒性影响增加。

Research of Toxicity Effect about Environmental Toxicants to the Staphylococcus Aureus

In order to determine the applicability of microsatellite primers developed from common carp (Cyprinus carpio) for genomic analysis in grass carp (Ctenopharyngodon idellus), twentyeight pairs of common carp primers designed for microsatellites containing CA motifs were employed to amplify the microsatellite loci in the genome of grass carp. The conditions of polymerase chain reaction (PCR) were optimized for the fidelity of DNA synthesis during PCR amplification. Two kinds of nonspecific PCR products, heteroduplex bands and shadow bands, were eliminated successfully by decreasing the extension temperature and Mg2+ concentration. 7 primers (about 25%) amplified specific products successfully and 4 primers (about 14.3%) have shown the polymorphism in a small population of the wild grass carp of xiangiang river (only 8 samples of fish). This result indicates that there are about 50% homology of sequences flanking the microsatellite loci between the common carp and grass carp, and some of the common carp