| 加热对IgG结合DNA能力的影响 |
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| 引用本文： | 梁俊峰,郑昌学.加热对IgG结合DNA能力的影响[J].中国生物化学与分子生物学报,1996,12(2):246-248. |
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| 作者姓名： | 梁俊峰 郑昌学 |
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| 作者单位： | 清华大学生物科学与技术系 |
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| 摘 要： | 加热对ＩｇＧ结合ＤＮＡ能力的影响梁俊峰，郑昌学（清华大学生物科学与技术系，北京１０００８４）聚集免疫球蛋白Ｇ（ＡｇｇｒｅｇａｔｅｄｌｇＧ）是指在某些条件（如温度、自由基等）影响下，ＩｇＧ自身聚集所形成的ＩｇＧ的多聚体．聚集不仅影响ＩｇＧ的免疫反应活性...
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| 收稿时间： | 1996-04-20 |
Heat-aggregated IgG Bind to DNA |
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| Liang Jun-Feng,Zheng Chang-Xue.Heat-aggregated IgG Bind to DNA[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(2):246-248. |
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| Authors: | Liang Jun-Feng Zheng Chang-Xue |
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| Institution: | (Department of Biological Sciences and Biotechnology,Tsinghua University, Beijing 100084 |
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| Abstract: | Aggregated immunoglobulin G were prepared by heat treatment of human IgG in vitro and the binding of these heat-aggregated IgG to DNA were studied by ELISA. It was found that heat-aggregated IgG,in comparison with normal IgG,are able to interact with DNA. Long-term incubation and high temperature in the range of 25─50℃ favored the yield of IgG with binding capacity to DNA. The experimental results obtained from CD spectra demonstrated that the conformation of heat-aggregated IgG is different from that of normal IgG,and that the interaction of heat-aggregated IgG with DVA can cause the change of DNA secondary structure. |
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| Keywords: | Immunoglobulin G Heat-aggregation DNA Interaction |
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