Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor |
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Authors: | Sang Jun Sim Ho Nam Chang |
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Affiliation: | (1) Bioprocess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 305-701 Daeduk Science Town, Taejon, Korea |
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Abstract: | Summary Plant hairy root cultures of Lithospermum erythrorhizon were carried out to produce shikonin derivatives by employing in situ extraction with n-hexadecane in a shake flask and a bubble column bioreactor. Over 95 % shikonin produced was recovered in the n-hexadecane layer. In flask cultures the maximum concentration of shikonin with n-hexadecane extraction was 3 times higher than that obtained without extraction. In the two phase bubble column reactor, 572.6 mg/L of shikonin and 15.6 g/L of dry cell mass were obtained after 54 days. Shikonin was produced at a constant level of 10.6 mg/L day during this period. |
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