Revealing
the F_actin Networks in Interphase Nuclei of Garlic Clove
Cells by Confocal Fluorescence Microscopy |
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Authors: | WANG Dong-Mei WANG Xue-Chen ZHANG Wei-Cheng |
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Abstract: | The interphase nuclei of parenchyma cells and epidermal cells of garlic (Allium sativum L.) clove were labelled with rabbit anti-actin antibody and FITC-conjugated goat anti-rabbit IgG antibody. The authors observed results with fluorescence microscopy and confocal laser scanning microscopy. The nuclei showed prominent green-yellow fluorescence, indicating the presence of actin in the nuclei. Fluorescence examination with TRITC-phalloidin showed distinctive red fluorescence in the nuclei, indicating that F-actin is present in the nuclei. Confocal laser scanning microscopy indicated the presence of F-actin containing network structures in the nuclei, but the network structures were absent and the nuclei still showed red fluorescence when the cells were treated with cytochalasin D before fixation; the red fluorescence in the nuclei was hard to be observed when the cells were treated with unlabelled phalloidin before the cells were stained with TRITC-phalloidin. These results indicate that F-actin is in the nuclei and forms network structures in the nuclei of garlic cells. |
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Keywords: | interphase nucleus F-actin TRITC-phalloidin cytochalasin D confocal laser scanning microscopy Allium sativum |
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