Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur |
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| Authors: | Marcia Berrêdo-Pinho Dario E Kalume Paloma R Correa Leonardo HF Gomes Melissa P Pereira Renata F da Silva Luiz RR Castello-Branco Wim M Degrave Leila Mendonça-Lima |
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| Affiliation: | 1.Laboratório de Gen?mica Funcional e Bioinformática,Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil,Rio de Janeiro,Brazil;2.Laboratório Interdisciplinar de Pesquisas Médicas,Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil,Rio de Janeiro,Brazil;3.Centro de Pesquisas Arlindo de Assis,Funda??o Ataulpho de Paiva (FAP),Rio de Janeiro,Brazil |
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| Abstract: | Background Bacille Calmette-Guerin (BCG) is currently the only available vaccine against tuberculosis (TB) and comprises a heterogeneous family of sub-strains with genotypic and phenotypic differences. The World Health Organization (WHO) affirms that the characterization of BCG sub-strains, both on genomic and proteomic levels, is crucial for a better comprehension of the vaccine. In addition, these studies can contribute in the development of a more efficient vaccine against TB. Here, we combine two-dimensional electrophoresis (2DE) and mass spectrometry to analyse the proteomic profile of culture filtrate proteins (CFPs) from M. bovis BCG Moreau, the Brazilian vaccine strain, comparing it to that of BCG Pasteur. CFPs are considered of great importance given their dominant immunogenicity and role in pathogenesis, being available for interaction with host cells since early infection. |
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