Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis |
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| Authors: | Dongjuan Dai Diane Holder Lutgarde Raskin Chuanwu Xi |
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| Affiliation: | (1) Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI 48109, USA;(2) Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, USA;(3) Proficiency and Validation Services Section, USDA, APHIS, FADDL, Greenport, NY, USA |
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| Abstract: | Background The study of bacterial species interactions in a mixed-species community can be facilitated by transcriptome analysis of one species in the community using cDNA microarray technology. However, current applications of microarrays are mostly limited to single species studies. The purpose of this study is to develop a method to separate one species, Escherichia coli as an example, from mixed-species communities for transcriptome analysis. |
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