Further evidence for entry of infectious bovine rhinotracheitis virus into bovine kidney cells |
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Authors: | Luciano Tiangco Talens Yuan Chung Zee |
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Institution: | (1) Department of Plant Pathology, College of Agriculture, University of the Philippines, Los Banos Unit College, Laguna, Philippines;(2) Department of Veterinary Microbiology, School of Veterinary Medicine, University of California, 95616 Davis, California, USA |
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Abstract: | The penetration of bovine kidney cells by infectious bovine rhinotracheitis virus, a member of the herpesvirus group, was
investigated using the direct immunoferritin labeling technique. Electron microscopic examination of infected cells after
10 min at 37°C revealed fusion between viral envelope and cell membrane; the former reacted with the ferritin particles conjugated
with antiviral antibody. However, shortly after penetration of the nucleocapsid, viral-specific antigenic sites on the plasma
membrane were not detected by the immunoferritin technique. Antigenically reactive structures in a disorganized array were
frequently detected extracellularly, situated above the penetration sites as indicated by the internalized nucleocapsids. |
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