EGTA inhibits floral induction of Pharbitis nil via its influence on gas exchange properties of stomata |
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| Authors: | Katarzyna Głowacka Andrzej Tretyn Ryszard Józef Górecki Sung-Ho Lee |
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| Affiliation: | (1) Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1a, 10-719 Olsztyn, Poland;(2) Department of Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Toruń, Poland;(3) Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701 Jinju, Korea |
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| Abstract: | The purpose of the study was to determine inhibitory effect of calcium chelator; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) on flowering of a short-day (SD) plant Pharbitis nil. It was found that 20 mM solution of EGTA applied on cotyledons of 5-d-old P. nil seedlings four hours before the start of 16-h-long induction night decreased the flowering response by 55% compared to the control plants not treated with this Ca2+ chelator. It also caused a very significant decrease of photosynthesis rate, transpiration rate and stomatal conductance both in light and darkness conditions. The results of this study confirm earlier hypothesis suggesting the effect of Ca2+ and its modulators on P. nil flowering is due to their influence on the stomata. |
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| Keywords: | calcium ions EGTA flowering Pharbitis nil photoperiodic induction short day plant stomatal conductance |
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