Formation of phenylethanoid glycosides by Cistanche deserticola callus grown on solid media |
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Authors: | Ouyang Jie Wang Xiao-dong Zhao Bing Wang Yu-chun |
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Institution: | (1) State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, China |
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Abstract: | The optimal growth of Cistanche deserticola callus and formation of phenylethanoid glycosides (PeG) was at 25°C with light irradiation intensity of 24 mol m–2 s–1 on solidified B5 media supplemented with 0.5 mg 6-benzylaminopurine l–1, 10 mg gibberellin l–1, 800 mg casein hydrolysate l–1 and 20 g sucrose l–1. After 30 d culture, the biomass reached 15.5 g dry wt callus l–1 medium and its PEG content was 10.7% (w/w). The PeG content was 42%–127% higher than those in explants. |
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Keywords: | callus growth Cistanche deserticola phenylethanoid glycosides |
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