Sequential liquid-liquid extraction of d-amino acid oxidase from Trigonopsis variabilis |
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| Authors: | Puneet Singh Neelam Verma Satwinder Singh Marwaha |
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| Affiliation: | (1) Department of Biotechnology, Punjabi University, Patiala, 147 002, India;(2) Biotechnology Division, PSCST, PB: 727, Sector 26, Chandigarh, 160 019, India |
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| Abstract: | A method for isolation of d-amino acid oxidase (DAAO) from disrupted Trigonopsis variabilis cells has been developed. In an aqueous two-phase system consisting of PEG6000 (220 g l–1), potassium phosphate (110 g l–1, K2HPO4 + KH2PO4 = 10.1:1, mol mol–1) and dl-methionine (11 g l–1), the major portion of cellular proteins (87%) was partitioned into the salt phase. By sequential extraction, 48% of DAAO was recovered in PEG phase, giving a yield of 211 U mg protein–1. |
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| Keywords: | aqueous two-phase system d-amino acid oxidase Trigonopsis variabilis |
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