壳聚糖酶结构与功能的研究进展

壳聚糖酶是一类对壳聚糖具有较高催化活性而几乎不水解几丁质的糖苷水解酶，其可将高分子量的壳聚糖转化为低分子量的功能性壳寡糖。近年来，对壳聚糖酶的相关研究取得了显著进展，因此，本文对其生化性质、晶体结构、催化机制和蛋白质工程改造进行总结和探讨，并对酶法制备壳寡糖纯品进行展望，这将加深研究者对壳聚糖酶作用机制的认识，推动壳聚糖酶的工业应用。

Advances in the structure and function of chitosanase

Chitosanases represent a class of glycoside hydrolases with high catalytic activity on chitosan but nearly no activity on chitin. Chitosanases can convert high molecular weight chitosan into functional chitooligosaccharides with low molecular weight. In recent years, remarkable progress has been made in the research on chitosanases. This review summarizes and discusses its biochemical properties, crystal structures, catalytic mechanisms, and protein engineering, highlighting the preparation of pure chitooligosaccharides by enzymatic hydrolysis. This review may advance the understandings on the mechanism of chitosanases and promote its industrial applications.