Molecular Threading: Mechanical Extraction,Stretching and Placement of DNA Molecules from a Liquid-Air Interface |
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| Authors: | Andrew C Payne Michael Andregg Kent Kemmish Mark Hamalainen Charlotte Bowell Andrew Bleloch Nathan Klejwa Wolfgang Lehrach Ken Schatz Heather Stark Adam Marblestone George Church Christopher S Own William Andregg |
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| Institution: | 1. Wyss Institute, Harvard University, Boston, Massachusetts, United States of America.; 2. Halcyon Molecular, Inc., Redwood City, California, United States of America.; 3. Biophysics Program and Wyss Institute, Harvard University, Boston, Massachusetts, United States of America.; 4. Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America.; Université d''Evry val d''Essonne, France, |
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| Abstract: | We present “molecular threading”, a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers. |
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