Characterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides Isolated from Boza,and Comparison of its Efficiency to Pediocin PA-1 |
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| Authors: | Kahina Maya Makhloufi Alyssa Carré-Mlouka Jean Peduzzi Carine Lombard Carol Ann van Reenen Leon Milner Theodore Dicks Sylvie Rebuffat |
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| Institution: | 1. Communication Molecules and Adaptation of Microorganisms (MCAM), UMR 7245 CNRS-MNHN, Muséum National d’Histoire Naturelle, Paris, France.; 2. Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.; Teagasc Food Research Centre, Ireland, |
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| Abstract: | A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EIIt
Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1. |
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