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MicroRNA 181 Suppresses Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection by Targeting PRRSV Receptor CD163 |
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| Authors: | Li Gao Xue-kun Guo Lianghai Wang Qiong Zhang Ning Li Xin-xin Chen Yongqiang Wang Wen-hai Feng |
| Affiliation: | State Key Laboratory of Agrobiotechnology,a;Ministry of Agriculture, Key Laboratory of Soil Microbiology,b;Department of Microbiology and Immunology,c;Department of Molecular Biology, College of Biological Sciences,d;Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine,e China Agricultural University, Beijing, China |
| Abstract: | We previously showed that microRNA 181 (miR-181) can inhibit PRRSV replication by directly targeting its genomic RNA. Here, we report that miR-181 can downregulate the PRRSV receptor CD163 in blood monocytes and porcine alveolar macrophages (PAMs) through targeting the 3′ untranslated region (UTR) of CD163 mRNA. Downregulation of CD163 leads to the inhibition of PRRSV entry into PAMs and subsequently suppresses PRRSV infection. Our findings indicate that delivery of miR-181 can be used as antiviral therapy against PRRSV infection. |
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