Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids |
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| Authors: | Javier Abadia John N Nishio Norman Terry |
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| Institution: | (1) Department of Plant and Soil Biology, University of California, 94720 Berkeley, CA, USA;(2) Present address: Estacion Experimental de Aula Dei, (C.S.I.C.), Ap. 202, Zaragoza, Spain |
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| Abstract: | The chlorophyll-protein and polypeptide composition of manganese deficient and control sugar beet thylakoids was examined using three different detergent-electrophoresis systems. On a per chlorophyll basis, manganese deficiency reduced the amounts of CPa complex (separated by sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis), and CP 47 and CP 43 complexes (separated by octylglucoside/SDS-polyacrylamide gel electrophoresis) without decreasing the amounts of light harvesting complexes. Lithium dodecylsulfate/Triton X-100 polyacrylamide gel electrophoresis showed that manganese deficiency decreased several thylakoid polypeptides, including a chlorophyll b containing 30 kilodalton chlorophyll-protein complex, but did not decrease the amounts of 28 and 29 kilodalton light-harvesting chlorophyll b-containing polypeptides. |
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| Keywords: | chlorophyll-protein complexes manganese deficiency photosystem II |
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