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Continuous-flow enrichment of a strain ofErwinia carotovora having specificity for highly methylated pectin
Authors:M. Leticia Almengor-Hecht  Alan T. Bull
Affiliation:(1) 30 calle 15-31, Zona 12, Guatemala City, C.A.;(2) Department of Applied Biology, University of Wales Institute of Science and Technology, CF1 3NU Cardiff, Wales, UK
Abstract:A pectinolytic bacterium was isolated from a mixed microbial population by means of a chemostat enrichment procedure. The bacterium, which was identified asErwinia carotovora, grew only on highly methylated pectin and produced a pectin lysase which released unsaturated monomer and dimer from 71% esterified citrus pectin. The pectin lyase was inducible only by pectins having a high methyl content and in pectin-limited chemostats its synthesis passed through a maximum at a dilution rate close to 0.04h-1.
Keywords:Chemostat enrichment  Erwinia carotovora  Pectin lyase
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