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| 角质细胞生长因子2基因(KGF2)的克隆及其对油菜的遗传转化 | |
| 引用本文： | 潘国庆,张爽,刘秀明,李营,张耀方,李洪志,李海燕,李校堃.角质细胞生长因子2基因(KGF2)的克隆及其对油菜的遗传转化[J].生物工程学报,2010,26(6):767-771. |
| 作者姓名： | 潘国庆 张爽 刘秀明 李营 张耀方 李洪志 李海燕 李校堃 |
| 作者单位： | 1. 吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118;吉林农业大学生命科学学院,长春130118 2. 中国人民解放军军事医学科学院军事兽医研究所,长春,130062 3. 吉林农业大学生物反应器与药物开发教育部工程研究中心,长春,130118 |
| 基金项目： | 国家高技术研究发展计划 (863计划) 生物反应器重大专项 (No. 2007AA100503)，吉林省和长春市科技发展计划项目 (Nos. 20070922, 06GG150)，高等学校科技创新工程重大项目培育资金 (No. 70S018) 资助。 |
| 摘 要： | 近年来,植物表达人源蛋白的研究逐渐增多。本研究以人角质细胞生长因子(KGF2)基因cDNA为基础,根据植物偏好密码子进行改造,并利用PCR方法合成KGF2基因全长cDNA。在此基础上构建了KGF2的植物油体系统表达载体p1390-YO-KGF2,并采用农杆菌介导法对甘蓝型油菜无菌苗的子叶进行转化。通过PCR、Southern杂交和Western blotting检测,证明外源基因KGF2已经转入油菜中并得到成功表达。 |
| 关 键 词： | 油菜，转基因植株，植物生物反应器，角质细胞生长因子 |
| 收稿时间： | 2009/12/13 0:00:00 |
Cloning of Keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L. |
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| Guoqing Pan,Shuang Zhang,Xiuming Liu,Ying Li,Yaofang Zhang,Hongzhi Li,Haiyan Li and Xiaokun Li.Cloning of Keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L.[J].Chinese Journal of Biotechnology,2010,26(6):767-771. | |
| Authors: | Guoqing Pan Shuang Zhang Xiuming Liu Ying Li Yaofang Zhang Hongzhi Li Haiyan Li and Xiaokun Li |
| Institution: | Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China; College of Life Science, Jilin Agricultural University, Changchun 130118, China;Veterinary Institute, Academy of Military Medical Science, Changchun 130062, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China |
| Abstract: | Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenicBrassica napus. |
| Keywords: | Brassica napus L transgenic plants plant bioreactor keratinocyte growth factor |
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