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Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs
Authors:Glenn A. Galau  D. Wayne Hughes  Leon Dure III
Affiliation:(1) Department of Botany, University of Georgia, 30602 Athens, GA, U.S.A.;(2) Department of Biochemistry, University of Georgia, 30602 Athens, GA, U.S.A.;(3) Department of Botany, University of Georgia, Miller Plant Sciences Building, 30602 Athens, GA, U.S.A.
Abstract:Summary Earlier studies found that cotton (Gossypium hirsutum L.) cotyledons contain several mRNAs which are more abundant during late embryogenesis than in mid-embryogenesis or early germination. They are here termed lsquoLate embryogenesis-abundantrsquo mRNAs, encoded by Lea loci. Complementary DNA clones for 18 such mRNA sequences, defined at a hybridization criterion of Tm-15°C, were identified in a mature embryo cDNA library by differential cDNA hybridization. At a lower hybridization criterion, some sequence homology was found within several of these cloned Lea mRNA sequences. Each Lea mRNA sequence comprises 0.04–1.3% of mature embryo poly(A)+ mRNA, a level ten-fold to several hundred-fold higher than in young embryo or 24 h seedling poly(A)+ mRNA. Of 18 Lea mRNA sequences examined in cultured young embryos, the level of at least 13 are specifically increased by exogenous abscisic acid (ABA), several to a level near that in normal mature embryos. However, the abundance of several of the sequences does not appear to be significantly modulated by ABA. The LEA polypeptides encoded by 10 Lea mRNA sequences were identified by hybrid-arrested translation. They include most of the late embryogenesis-abundant, ABA-inducible, polypeptides previously identified. Preliminary results suggest that many of the individual Lea mRNA sequences are transcribed from 1–3 genes in each of cotton's two subgenomes.
Keywords:embryo maturation  cDNA cloning  Gossypium hirsutum  hybrid-arrest translation  abscisic acid  embryo culture
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